Journal:

Article Title: Heteroduplex Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains

doi: 10.1128/AEM.67.9.4084-4090.2001

Figure Lengend Snippet: HPA panels of Aspergillus Section Flavi strains obtained by using ITS-region DNAs amplified by PCR. The left panels of reference strains P-39-1, IFO4082, A0754, and ATCC 15546 are the standard panels of F-1, P-1, T-1, and N-1, respectively. On the right are the test panels of 20 strains that are indicated above the panels. F-2, N-2, N-3, and TN-1 are additional new panels of heteroduplex types. Heteroduplex bands in lanes f, p, t, and n were formed with reference strains of A. flavus, A. parasiticus, A. tamarii, and A. nomius, respectively.

Article Snippet: Except for type TN-1, intraspecific diversity (1 to 3 bp) did not display any heteroduplex bands with mobility shifts (lane f of F-2 and lanes n of N-2 to N-3 in Fig. ), while other interspecific heteroduplexes (lanes p, t, and n of F-2 and lanes f, p, and t of N-2 and N-3 in Fig. ) displayed mobility shifts apparently different from those of the corresponding standard panels. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Type a Nucleotide diversity (no. of bases) from: F-1 (595) b F-2 (594) P-1 (595) T-1 (598) N-1 (598) N-2 (598) N-3 (598) TN-1 (597) F-1 0 F-2 1 0 P-1 8 9 0 T-1 13 14 9 0 N-1 20 21 14 13 0 N-2 19 20 13 12 2 0 N-3 19 20 13 12 1 3 0 TN-1 14 15 8 5 8 8 7 0 Open in a separate window a F-1, A. flavus P-39-1 and A. oryzae IFO 30113; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082 and A. sojae IFO 4200; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. b The numbers within parentheses indicate the sizes (base pairs) of ITS region DNAs amplified by PCR.

Techniques: Amplification

Journal:

Article Title: Heteroduplex Panel Analysis, a Novel Method for Genetic Identification of Aspergillus Section Flavi Strains

doi: 10.1128/AEM.67.9.4084-4090.2001

Figure Lengend Snippet: Nucleotide sequence alignments for ITS1 (A) and ITS2 (B) of rRNA genes from Aspergillus Section Flavi strains. F-1, A. flavus P-39-1; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. Dots indicate identity to the sequence of F-1. Dashes indicate alignment gaps (insertion or deletion differences). Boxes A to E indicate variable regions.

Article Snippet: Except for type TN-1, intraspecific diversity (1 to 3 bp) did not display any heteroduplex bands with mobility shifts (lane f of F-2 and lanes n of N-2 to N-3 in Fig. ), while other interspecific heteroduplexes (lanes p, t, and n of F-2 and lanes f, p, and t of N-2 and N-3 in Fig. ) displayed mobility shifts apparently different from those of the corresponding standard panels. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Type a Nucleotide diversity (no. of bases) from: F-1 (595) b F-2 (594) P-1 (595) T-1 (598) N-1 (598) N-2 (598) N-3 (598) TN-1 (597) F-1 0 F-2 1 0 P-1 8 9 0 T-1 13 14 9 0 N-1 20 21 14 13 0 N-2 19 20 13 12 2 0 N-3 19 20 13 12 1 3 0 TN-1 14 15 8 5 8 8 7 0 Open in a separate window a F-1, A. flavus P-39-1 and A. oryzae IFO 30113; F-2, A. oryzae IFO 5375; P-1, A. parasiticus IFO 4082 and A. sojae IFO 4200; T-1, A. tamarii A0754; N-1, A. nomius ATCC 15546; N-2, A. nomius NRRL 6552; N-3, A. nomius IMI 358751; TN-1, A. nomius IMI 358749. b The numbers within parentheses indicate the sizes (base pairs) of ITS region DNAs amplified by PCR.

Techniques: Sequencing

Journal: Contrast Media & Molecular Imaging

Article Title: Key Parameters on the Microwave Assisted Synthesis of Magnetic Nanoparticles for MRI Contrast Agents

doi: 10.1155/2017/8902424

Figure Lengend Snippet: MRI T 1 and T 2 relaxation times as a function of the Fe concentration for DMSA coated magnetite nanoparticle suspensions obtained by microwave (MW) and thermal decomposition (TD) using different solvents.

Article Snippet: Finally, MRI relaxometric properties were investigated by measuring the longitudinal ( T 1 ) (sequence t1_ir_mb) and transversal ( T 2 ) (sequence t2_ir_mb) protons relaxation times at different dilutions between 0 and 0.07 mM of Fe in a MINISPEC MQ60 (Bruker) at 37°C and a magnetic field of 1.5 T. The sequences used are original from Bruker.

Techniques: Concentration Assay

Journal:

Article Title: Regulation of the Cellulosomal celS ( cel48A ) Gene of Clostridium thermocellum Is Growth Rate Dependent

doi: 10.1128/JB.185.10.3042-3048.2003

Figure Lengend Snippet: RPA for celS-initiated mRNA derived from cellulose-grown C. thermocellum cells. RPAs were performed using RNA from exponential- or stationary-phase cultures grown on crystalline cellulose. Cell samples were harvested and snap-frozen in liquid nitrogen, and total RNA was extracted using the RNeasy kit from Qiagen. Different amounts of RNA (indicated in micrograms above each lane) were hybridized overnight with 50,000 cpm of 32P-labeled 428-nt antisense celS probe, and then digested with RNase A-RNase T1 (Promega) for 30 min. The protected RNAs were placed directly in a scintillation counter for quantification and then separated on a 5% polyacrylamide gel containing 7 M urea. The radiolabeled bands were visualized using a phosphorimager system. The expected size of the protected products was 380 nt. (Top) Representative autoradiograph of the protected products subjected to phosphorimager analysis. (Bottom) Correlation between the amount of total RNA used in the assay and the counts obtained for the protected products. The negative control (lane C) contained yeast RNA instead of C. thermocellum RNA. The full-length probe was used in lane P. The graphs represent the average values of at least three separate experiments, and the experimental error was ±15%.

Article Snippet: Different amounts of RNA (indicated in micrograms above each lane) were hybridized overnight with 50,000 cpm of 32 P-labeled 428-nt antisense celS probe, and then digested with RNase A-RNase T 1 (Promega) for 30 min.

Techniques: Derivative Assay, Labeling, Autoradiography, Negative Control